Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Tobacco Research Institute, Dr. N.C. Gopalachari Road, Bhaskar Nagar, Rajamundry-533105 , Andhra Pradesh (https://ctri.icar.gov.in/)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: SPECTROPHOTOMETER
Make                                   : Thermo scientific
Model                                  : ND1000
Specification   :

Click to ViewThe Thermo Scientific NanoDrop™1000 Spectrophotometer measures 1µl samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilize sap tented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvette sand other sample containment devices and allows for cleanup in seconds.

  Working Principles
Click to ViewSpectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution.

  Applications
  User Instructions
UV/VIS spectrophotometry is simple for samples as small as1µl using the NanoDrop1000 Spectrophotometer. • Nucleic acid concentration and purity of nucleic acid samples upto 3700 µg/ul (ds DNA) without dilution • Purified protein analysis (A280) upto100mg/ml (BSA) • Bradford Assay analysis of protein • BCA Assay analysis of protein • Lowry Assay analysis of protein • Pierce Protein 660nm Protein Assay • Cell density measurements • General UV-Vis spectrophotometry 1. Apply 5µl of d H20 solution on to each bottom pedestal. 2. Lower the up pedestal arm to form a liquid column; let it sit for approximately 2-3 minutes. 3. Wipe away the water from both the upper and lower pedestal with a clean lab wipe and 1µl of sample can be loaded for measurement.

  Contact Us
Contact No. 0883 2449871-74
Email Id ctri-ap@nic.in

  SPECTROPHOTOMETER Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""ICAR-CTRI, Rajahmundry"
Letter, DD & Samples send to ""DIRECTOR, ICAR-CTRI, Bhaskar Nagar, Rajahmundry - 533105 Andhra Pradesh, India " "