Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, MRC Nagar, Chennai-600028 Tamil Nadu (www.ciba.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Scanning Electron Microscope
Make                                   : JEOL
Model                                  : JSM IT300
Specification   :

Click to ViewImage analysis takes one day.

  Working Principles
Click to ViewThe Scanning Electron Microscope (SEM) utilizes electrons to show an enlarged image of a specimen, in the same way as the transmission electron microscope (TEM). Since an electron has a shorter wavelength than light, it enables us to observe smaller things than can be seen with the Optical microscope. The word resolution refers to the smallest size that can clearly be observed (the resolution of the human eye is said to be 0.2mm). The SEM’s resolution is 0.5 to 4nm. SEM provides an enlarged image projected onto a fluorescent screen, by using the electrons which were reflected or generated from the surface of the specimen whereas in TEM the image is formed by the electrons transmitted (passed) through the thinly prepared specimen.

  Applications
  User Instructions
The JSM IT300 integrated with secondary electron detector that makes it ideal for studying the samples in biology, taxonomy, anatomy and pathology. The resolution of SEM is 0.5 to 4nm. CIBA has expertise on scanning electron microscopy of biological samples and encourages SEM studies related to biological samples.The samples should be dry and should withstand high vacuum (~ 10 -5 Pa). Users must prepare their samples using appropriate protocols to ensure that the native structure of the organism is retained. Users should care to avoid the introduction of drying artifacts, shrinkage and collapse that can be seen in microphotographs of poorly preserved / processed specimens. Users must take effort to do some research prior to collecting samples and make SEM work easier. Fixation is the first and most important step for optimum preservation of biological samples. Make sure to use the best fixative for collecting and preserving your samples. The sample size for dry samples should be less than diameter: 100 mm Height: 40 mm. Wet biological sample withstand low temperature (-10 ◦C – 20 ◦C) can be analysed. The user is welcome to be present in the laboratory during analysis.

  Contact Us
Contact No. 91-044-24610565
Email Id sathishkumart@ciba.res.in

  Scanning Electron Microscope Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
7000
3000
3000
Image analysis per sample

Additional Rs 100/-towards postal charges if result are to be posted
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Letter, DD & Samples send to ""