Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Plantation Crops Research Institute, Kudlu P.O., Kasaragod-671124, Kerala (www.cpcri.icar.gov.in)
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Instrument Details
Instrument/Facility Name: Soxhlet Apparatus
Make                                   : Rotek
Model                                  : Six Mantles
Specification   :A Soxhlet extractor is a piece of laboratory apparatus designed for the extraction of a lipid from a solid material.

Click to ViewA Soxhlet extractor is a piece of laboratory apparatus designed for the extraction of a lipid from a solid material. Typically, a Soxhlet extraction is used when the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent. It allows for unmonitored and unmanaged operation while efficiently recycling a small amount of solvent to dissolve a larger amount of material.

  Working Principles
Click to ViewA flask containing solvent is heated to vaporize the solvent which runs along the sidearm of the Soxhlet until it reaches the condenser. The vapors condense and drip into the thimble in the Soxhlet extractor which should contain the material to be extracted. Ideally, the material to be extracted should be ground as fine as possible to enable the desired compound to easily be removed. Over time, sufficient solvent will have evaporated from the flask and condensed to fill the Soxhlet extractor. Once the Soxhlet is filled, the liquid contents will empty back into the solvent flask along with any compounds which are dissolved in the solvent. Insoluble solids are left behind in the thimble. The process will continue to cycle, with the solvent evaporating from the flask and refilling the Soxhlet. Over time all the soluble component(s) will be extracted into the round-bottomed flask. The compounds can then be isolated by rotary evaporation

  Applications
  User Instructions
Soxlet extraction is to obtain compounds from leaves, berries, seeds or fruit; Fat and Oil estimation.Normally a solid material containing some of the desired compound is placed inside a thimble made from thick filter paper, which is loaded into the main chamber of the Soxhlet extractor. The Soxhlet extractor is placed onto a flask containing the extraction solvent. The Soxhlet is then equipped with a condenser.The solvent is heated o reflux. The solvent vapour travels up a distillation arm, and floods into the chamber housing the thimble of solid. The condenser ensures that any solvent vapour cools, and drips back down into the chamber housing tbe solid material.The chamber containing the solid material slowly fills with warm solvent. Some of the desired compound will then dissolve in the warm solvent. When the Soxhlet chamber is almost full, the chamber is automatically emptied by a siphon side arm, with the solvent running back down to the distillation flask. This cycle may be allowed to repeat many times, over hours or days. During each cycle, a portion of the non-volatile compound dissolves in the solvent. After many cycles the desired compound is concentrated in the distillation flask. The advantage of th is system is that instead of many portions of warm solvent bemg passed through thesample,just one hatch of solvent is recycled. After extraction the solvent is removed, typically by means of a rotary evaporator, yielding the extracted compound. The non-soluble portion of the extracted solid remains in the thimble, and is usually discarded. Prior confirmation may be obtained from the Institute before taking DD / sending samples.

  Contact Us
Contact No. 04994232090
Email Id director.cpcri@icar.gov.in

  Soxhlet Apparatus Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Soxhlet Analysis
500
250
250
Prior confirmation may be obtained from the Institute before taking DD / sending samples.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Unit-CPCRI Kasaragod"
Letter, DD & Samples send to "The Director, ICAR-CPCRI, Kudlu P.O Kasaragod - 671 124. Kerala"