Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Plantation Crops Research Institute, Kudlu P.O., Kasaragod-671124, Kerala (www.cpcri.icar.gov.in)
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Instrument Details
Instrument/Facility Name: Spectrophotometer
Make                                   : Shimadzu
Model                                  : UV1601
Specification   :The Shimadzu UV-1601 PC is a UV/Visible double beam spectrophotometer. It provides high performance with an optical bench that is equipped with a monochromator allowing for trouble-free operation.

Click to View• Lighting: 50W halogen lamp (long-life 2000 hour), Deuterium lamp (sockettype), On-board automatic light source positioning mechanism. • Stray Light: Photometric System: double beam optics • Wavelength Range: 190-1100 nm • Operating Conditions: Room temperature 15-35°C, Humidity 45-80% • Bandwidth: 2 nm • Scanning Speed: 3200nm/min to 160nm/min • Detector: Silicon photodiode • Optical System: Monochromator uses aberration-correcting concave blazed holographic grating • Photometric Range: Absorbance of -0.5~3.999 Abs / Transmittance of 0.0~300% • Sample Compartment: Interior dimensions: 110Wx230Dx105H (mm) • Distance between beams: 100mm • Removable type 2-screw attachment Beam dimensions: 1x10mm (WxH) (at center of sample compartment) • Additional Specifications Wavelength Accuracy: +/- 0.5 nm • Wavelength Reproducibility: +/- 0.1 nm • Wavelength Display: readable to 0.1 nm • Wavelength Setting: 0.1 nm increments • Wavelength Slew Rate: about 6000nm/min • Photometric Accuracy: +/- 0.004 Abs at 1.0 Abs • UV Probe Software not included

  Working Principles
Click to View1. Ultraviolet–visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. This means it uses light in the visible and adjacent (near-UV and near-infrared (NIR)) ranges. 2. UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. 3. UV/Vis spectrophotometer measures the intensity of light passing through a sample (I), and compares it to the intensity of light before it passes through the sample (I0). The ratio is called the transmittance, and is usually expressed as a percentage 4. The instrument works based on Beer-Lamberts law.

  Applications
  User Instructions
Spectrophotometer is commonly used for measuring the concentration of coloured as well as colourless compounds by measuring the optical density or its absorbance. It can be used for the determination of course of the reaction by measuring the rate of formation and disappearance of the light absorbing compounds in the range of the UV-Visible range of the electromagnetic spectrum. By spectrophotometer, a compound can be identified by determining the absorption spectrum in the visible region of the light spectrum as well as the UV region of the electromagnetic spectrum. 1. Check the power supply before start. 2. Set correct wavelength for the element to measure 3. Check the standard concentration used. 3. Follow the steps in the user manual.

  Contact Us
Contact No. 04994232090
Email Id director.cpcri@icar.gov.in

  Spectrophotometer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Spectrophotometry
1000
500
500
Prior confirmation may be obtained from the Institute before taking DD / sending samples.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Unit-CPCRI Kasaragod"
Letter, DD & Samples send to "The Director, ICAR-CPCRI, Kudlu P.O Kasaragod - 671 124. Kerala"