Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Inland Fisheries Research Institute, PO Monirampare, Barrackpore, Kolkata-700120 West Bengal (www.cifri.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: TWO DIMENSIONAL GEL ELECTROPHORESIS SYSTEM
Make                                   : Amersham Biosciences
Model                                  : Ettan IPGphor 3 IEF system (for first dimension gel electrophoresis) & SE 600 vertical unit (for second dimension gel elctrophoresis).
Specification   :A fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2DE. In our institute, we can run up to 12 IPG strips (7 or/& 11 cm) simultaneously using Ettan IPGphor manifold or 02 strips using ceramic strip holders in single time. Ettan IPGphor 3 Control Software allows real time monitoring of the all first dimensional run with similar set of run parameters. Focussed proteins are resolved on medium size slab gel using SE 600 vertical unit (Amersham biosciences).

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  Working Principles
Click to ViewTwo-dimensional gel electrophoresis (or 2DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique separate proteins in two steps: the first-dimension gel electrophoresis is isoelectric focusing (IEF), which separates proteins according to their isoelectric points (pI); the second-dimension is SDS-polyacrylamide gel electrophoresis (SDS-PAGE), which separates proteins according to their molecular weights (MW). In this way, complex mixtures consisted of thousands of different proteins can be resolved and the relative amount of each protein can be determined. Two-dimensional gel electrophoresis (2-DE) is an important tool for proteomics. The 2-DE can achieve the separation of several thousand different proteins in one gel. Differentially expressed proteins can be subsequently identified by mass spectrometric methods.

  Applications
  User Instructions
Comparative proteomics researchThe cells, tissues, or other biological samples (~1.0 gm in weight) or pure/crude protein samples should be in perfectly cold conditions (dry ice/liquid nitrogen/ice packs/ice). Avoid repeated change in storage conditions of sample(s). All samples should be freshly dissected/prepared in standard conditions.

  Contact Us
Contact No. 033 25920177
Email Id director.cifri@gmail.com

  TWO DIMENSIONAL GEL ELECTROPHORESIS SYSTEM Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Praveen Maurye
Working condition

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit, CIFRI, Barrackpore"
Letter, DD & Samples send to ""