Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Citrus Research Institute, Post Box No.464, Shankar Nagar P.O., Nagpur-440 010 (www.ccringp.org.in)
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Instrument Details
Instrument/Facility Name: Thermal Cycler - PCR
Make                                   : BIO-RAD
Model                                  : T100TM
Specification   :The T100 thermal cycler is a small thermal cycler offering a comprehensive set of convenient features in a small footprint.

Click to ViewThis is compact thermal cycler features an intuitive touch-screen user interface • Sample capacity - 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate • Maximum ramp rate, °C/sec - 4 • Average ramp rate, °C/sec - 2.5 • Input power - 100–150 VAC, 50–60 Hz; 220–240 VAC, 50–60 Hz; 700 W maximum

  Working Principles
Click to ViewPCR uses the enzyme DNA polymerase that directs the synthesis of DNA from deoxynucleotide substrates on a single-stranded DNA template. DNA polymerase adds nucleotides to the 3` end of a custom-designed oligonucleotide. The reaction consists of three steps denaturation, annealing and extension which are repeated one after another for 20 to 30 cycles in thermalcycler. The end product of the technique is amplified DNA which can be checked using agarose gel electrophoresis.

  Applications
  User Instructions
PCR allows isolation of DNA fragments from genomic DNA by selective amplification of a specific region of DNA. This use of PCR augments many ways, such as generating hybridization probes for Southern or northern hybridization and DNA cloning, which require larger amounts of DNA, representing a specific DNA region. PCR supplies these techniques with high amounts of pure DNA, enabling analysis of DNA samples even from very small amounts of starting material. Other applications of PCR include DNA sequencing to determine unknown PCR-amplified sequences in which one of the amplification primers may be used in Sanger sequencing, isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into a plasmid, phage the genetic material of another organism. PCR may also be used for genetic fingerprinting; a forensic technique used to identify a person or organism by comparing experimental DNAs through different PCR-based methods. PCR amplifies the regions of targeted DNA thus it can be used to analyze extremely small amounts of sample.Sample should be clean and contamination free

  Contact Us
Contact No. 0712- 2500249, 2500813 Ext- 139
Email Id ghoshdk@hotmail.com

  Thermal Cycler - PCR Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""