Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal-462022 Madhya Pradesh (www.nihsad.nic.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Transmission Electron Microscope
Make                                   : Jeol
Model                                  : JEM-1400
Specification   :120 kV class high-resolution transmission electron microscope

Click to ViewEM unit of NIHSAD has a 120 kV class high-resolution transmission electron microscope (JEM-1400, Jeol, Japan). The unit also has an ultramicrotome (Ultracut-UCT, Leica) and a knife maker (EMKMR2, Leica) for ultra-thin sectioning of tissue samples. The services of the TEM unit add to the existing array of diagnostic facilities available at NIHSAD for quick confirmation of various exotic and emerging viral infections of animals. Apart from being useful in ultrastructural studies on viral infections, it can be of great help in identification and confirmation of viral etiology in case of unknown disease outbreaks.

  Working Principles
Click to ViewTransmission electron microscopy uses high energy electrons which are accelerated to nearly the speed of light. The electron beam behaves like a wave-front with wavelength about a million times shorter than light waves. When an electron beam passes through a ultrathin-section specimen of a material, electrons are scattered. A sophisticated system of electromagnetic lenses focuses the scattered electrons into an image on a phosphor-screen or charge coupled device (CCD) camara

  Applications
  User Instructions
Ultrastructure studies.• Guidelines for submission of samples is given in http://nihsad.nic.in/SAIF/charges.htm • Interpretation of data/results should be done by the user. • The analytical data/images are provided for research use only. They should not be used as certificates in legal disputes. • It is desirable that the work done using the facility be acknowledged in publications/thesis/dissertations and a note in this regard may be sent to us for record purpose. • Diffraction analysis is not performed at NIHSAD, Bhopal.

  Contact Us
Contact No. 07552750647
Email Id krajukr@yahoo.com

  Transmission Electron Microscope Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Specimen preparation (1 block per specimen; 2 LM slides per block; 2 grids stained for the best block)
3500
2000
2000
Non-governmental R&D labs will be charged at Industry rates
Negative staining/grid preparation of liquid samples (per grid)
1400
700
700
Non-governmental R&D labs will be charged at Industry rates
Immunolabelling (per sample per primary antibody (to be provided by user)
4000
2500
2500
Non-governmental R&D labs will be charged at Industry rates
Viewing and photography (per hour or part thereof) (new CDs to be brought by user)
1800
900
900
Non-governmental R&D labs will be charged at Industry rates

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT NIHSAD, BHOPAL payable at SBI, HET Branch, Bhopal."
Letter, DD & Samples send to "Director, ICAR-NIHSAD, Anand Nagar, Bhopal or Dr. K. Rajukumar, Principal Scientist and In-charge Electron Microscopy, ICAR-NIHSAD, Anand Nagar, Bhopal 462022 Madhya Pradesh"