Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Horticultural Research, Hassaraghatta Lake Post, Bengaluru- 560 089 Karnataka (www.iihr.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Transmission and Scanning Electron Microscope Facility
Make                                   : Manufactured by Hitachi Ltd, Japan With Power backup, computing and Imaging facilities.
Model                                  : HT7700 Transmission Electron Microscope,TM3030Plus Scanning Electron Microscope
Specification   :HT7700 Transmission Electron Microscope Magnification: ×50 - ×1,000, (low Mag mode) ×200 - ×200,000 (High Controst mode), ×4,000 - ×600,000 (High Resolution mode) Accelerating voltage: 40 - 120 kV (100 V/step variable) Image output: : 8 M pixel Main Camera 640 × 480/ 1,280 × 960/ 2,560 × 1,920/ 5,120 × 3,840 pixel,TIFF (8 or 16 bit) , BMP, JPEG images. With auto nano / micron marker Auto focus. Microtrace, Autodrive, Autophoto, features Auto-gun alignment, Live FFT display, Measurement function, Low dose and auto pre-irradiation facility Image output in CD/DVD.

Click to ViewTM3030Plus Scanning Electron Microscope 15 to 120,000x Signal select Observation mode 5 kV/15 kV, Back Scattered electrons / Secondary Electron or Mix (30 % BSE: 70% SE) detector With Conductor/ Standard/ Charge-up Reduction modes Maximum sample size 70 mm diameter.Auto start, Auto focus, Auto rightness/contrast Image Size:640x480 pixels, 1,280x960 pixels as BMP, TIFF, JPEG Auto Micron marker Turbomolecular pump Image output in CD/DVD.

  Working Principles
Click to ViewSample dimensions, Size distribution. Nano / Miro structure, morphological .

  Applications
  User Instructions
The Transmission Electron microscope can image viruses and other plant pathogens. It can also image metallic nano particles. upto 300000 times primary magnification This Variable Pressure Scanning Electron Microscope can be used to image structure of bio materials upto 60000 times primary magnification.For Transmission Electron microscope HT7700 1.Leaf Dip Preparations.The sap neds to be extracted and applied on TEm grids, After addition of PhosphoTungstic Acid or Uranyl Acetate stain, The sample needs to be washed and dried. This can be imged within a day. 2.Ultrathin sectioningThe sample needs to be fixed with Osmium tetroxide or Glutaraldehyde or Paraformaldehyde and then needs to be dehydrated in an alcohol series. Followed by Embeding in Epoxy resin blocks, The cured blocks would then be cut in a Ultramicrotome using glass blades. The cut sections ned to be in 40 – 50 na1nometers (nm) thick, The sections would need to be stained and imaged after drying. Time requirement will be 2 weeks. For Scanning Electron Microscope 1.As the Instrument has Variable Pressure Detector, the samples need to be stuck on 2 sided conducting adhesive copper tape on an aluminium stub. If needed a carbon coat can be done using the Vaccum evaporator to increase controst. Staining using Phospho Tungstic Acid neds to be done for higher magnifications. The imaging can be done immediately.

  Contact Us
Contact No. 080-28466420-23
Email Id pathology@iihr.res.in

  Transmission and Scanning Electron Microscope Facility Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Dr M. Krishna Reddy, Head, Division of Plant Pathology.
8000/-& 10
5000/- & 5
5000/- & 5

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "Director, ICAR-IIHR, Bengaluru"
Letter, DD & Samples send to "The payment should be made in advance by Demand Draft(DD) in favour of : "Director, ICAR-IIHR, Bengaluru" OR by NEFT transfer to IIHR account with following details. Beneficiary details: Indian Institute of Horticultural Research, Bank Account Number: 37578009241 , Name of the Bank : State Bank of India, Type of Account: Current Account, IFSC No.: SBIN0041187 ."