Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Horticultural Research, Hassaraghatta Lake Post, Bengaluru- 560 089 Karnataka (www.iihr.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: UPLC-MS/MS
Make                                   : WATERS Acquity UPLC H-Class
Model                                  : Coupled with Acquity-TQD MS/MS
Specification   :UPLC- Pressure can be hold up-to 15000 psi, with flow from 0.05 ml to 0.5 ml/min PDA Detector 190nm -900nm MS/MS Mass range scan 20amu to 2000 amu, with electro spray ionization of ESI+ and ESI-, APCI+ and APCI-

Click to ViewUPLC- Pressure can be hold up-to 15000 psi, with flow from 0.05 ml to 0.5 ml/min PDA Detector 190nm -900nm MS/MS Mass range scan 20amu to 2000 amu, with electro spray ionization of ESI+ and ESI-, APCI+ and APCI-

  Working Principles
Click to ViewProfiling and estimation of Phenolic acids, Flavonoids, Amino acids, Polyamines, Water and Fat soluble vitamins, Plant Hormones, Organic acids, Anthocyanins, Alkaloids, Saponins, Carotenoids, Sugars etc. Profiling and estimation of Phenolic acids, Flavonoids, Amino acids, Polyamines, Water and Fat soluble vitamins, Plant Hormones, Organic acids, Anthocyanins, Alkaloids, Saponins, Carotenoids, Sugars etc.

  Applications
  User Instructions
To measure the molecular wt. and their fragmentation of unknown compounds. Plants Metabolomics and phytochemicals identifications and quantification by injecting through UPLC or by direct insertion to MS/MS.Extraction should be done as per the standard published methodology for individual groups of compounds. A minimum of 2 ml of the extract can be sent for the LC-MS analysis. If solid samples (Plant samples/mushrooms only) are to be sent then a minimum of 100 g is needed.

  Contact Us
Contact No. 08028466420
Email Id tkr@iihr.res.in

  UPLC-MS/MS Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Mr. T.K.Roy, Chief technical Officer.
5000/-
1500/-
3000/-

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "Director, ICAR-IIHR, Bengaluru"
Letter, DD & Samples send to "The payment should be made in advance by Demand Draft(DD) in favour of : "Director, ICAR-IIHR, Bengaluru" OR by NEFT transfer to IIHR account with following details. Beneficiary details: Indian Institute of Horticultural Research, Bank Account Number: 37578009241 , Name of the Bank : State Bank of India, Type of Account: Current Account, IFSC No.: SBIN0041187 ."