Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Water Management, Opposite Rail Vihar, Chandrashekharpur, Bhubaneswar-751023 Odisha (http://www.iiwm.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: UV-VIS Sprectrophotometer
Make                                   : labomed inc
Model                                  : UVD-2960
Specification   :It is a two (2) cell spectrophotometer with a variable bandwidth of 0.5, 1.0, 2.0 and 5.0nm. With its focused-beam design, the system provides optimal and reproducible results for small samples. The sample beam and the reference beam are provided within the same sampling space, facilitating wider and longer scan of data providing a more detailed view of the results in an easy to use environment.

Click to ViewUVD-2960 has a large LCD screen which displays the menu screen and makes the device user friendly. Spectro UVD-2960 has 2 cell holder for reference (standard) and sample.

  Working Principles
Click to ViewThe Spectrophotometer is a much more refined version of a colorimeter. In a colorimeter, filters are used which allow a broad range of wavelengths to pass through, whereas in the spectrophotometer a prism (or) grating is used to split the incident beam into different wavelengths. By suitable mechanisms, waves of specific wavelengths can be manipulated to fall on the test solution. The range of the wavelengths of the incident light can be as low as 1 to 2nm. The spectrophotometer is useful for measuring the absorption spectrum of a compound, that is, the absorption of light by a solution at each wavelength. The visible and UV spectrophotometer may be used to identify classes of compounds in both the pure state and in biological preparations. This is done by plotting absorption spectrum curves. Absorption by a compound in different regions gives some hints to its structure. Spectrophotometer uses in the Quantitative analysis of Biochemistry practicals. Quantitative analysis method developing for determining an unknown concentration of a given species by absorption spectrometry. Most of the organic compounds of biological interest absorb in the UV-visible range of the spectrum. Thus, a number of important classes of biological compounds may be measured semi-quantitatively using the UV-visible spectrophotometer. Nucleic acids at 254nm protein at 280nm provide good examples of such use. The absorbance at 280nm by proteins depends on their "Tyrosine" and "Tryptophan" content.

  Applications
  User Instructions
Organic compound Food & Agricultural Industries. 1. Sample must be pure and all contaminants must be removed. 2. Sample weight should be in between 10-20 mg. 3. Liquid samples should have constant weight.

  Contact Us
Contact No. 06742300060
Email Id director.iiwm@icar.gov.in

  UV-VIS Sprectrophotometer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
3000
1500
1500

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT IIWM Bhubaneswar"
Letter, DD & Samples send to "Director, ICAR-IIWM, Bhubaneswar"