Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Institute of Post-harvest Engineering & Technology, Post Office PAU, Behind Radha Swami Satsang Bhawan, Humbran Road, Ludhiana-141004 Punjab (www.ciphet.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: UV/ visible spectrophotometer
Make                                   : SHIMADZU
Model                                  : UV-2550/
Specification   :Spectrophotometer having detection system both for ultraviolet (190-380) and visible (380-760 nm). In addition has detection limit to infrared region up to 900 nm.

Click to ViewSpectrophotometer having detection system both for ultraviolet (190-380) and visible (380-760 nm). In addition has detection limit to infrared region up to 900 nm.

  Working Principles
Click to ViewIt refers to absorption spectroscopy and based on Lambert-Beer’s law which states that when a beam of monochromatic light passes through a solution, absorbance of that solution is directly proportional to concentration of absorbing molecule/species and its path length at a particular wavelength.

  Applications
  User Instructions
1. Quantitative determination of minute quantities of substances like biochemical, pigments, metabolites, macromolecules of plants, microbes, soil etc. 2. Enzymes reactions and kinetics 3. Screening lambda max of various compounds and components 1. Turn on instrument and its software 2. Set wavelength, put blank in cuvette 3. Place cuvette in reference slot and do auto-zero 4. Pour clear sample in cuvette, place in sample slot and record absorbance which should be in the range of 0.0 to 1.0-1.3 5. Turn off first software and then instrument 6. Samples should be clear one

  Contact Us
Contact No. 01634-224024
Email Id rajeshkumarciphet@gmail.com

  UV/ visible spectrophotometer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Dr. Rajesh Kumar Vishwakarma, Principal Scientist
520
451
486
Per Sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""