Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), ICAR Campus, Ramagondanahalli, Yelahanka, Bengaluru-560064 Karnataka (www.nivedi.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Ultracentrifuge with accessories +
Make                                   : Sorvallâ„¢ Japan
Model                                  : MTX 150 Micro-Ultracentrifuge
Specification   :

Click to ViewThe Thermo Scientific Automatic Securing Micro-Ultracentrifuge Swinging Bucket and Vertical Rotors are for use in the Sorvall Discovery M150 Rotor Type: S 52 ST-0319 Tubes/Buckets: 4 (5 ml capacity) Speed: With good acceleration rate of 1,50,000 rpm which reaches in a short period of time. The range of RCF of 1,048, 000xg supports any separation. This benchtop ultracentrifuge offers powerful separation technology in a quiet, compact, easy to use benchtop design.

  Working Principles
Click to ViewThere are different techniques/methods used for separation of proteins/antigens/nucleic acids using different gradients. Continuous sucrose gradient is usually followed for separation of viral proteins/antigens. Different gradients of sucrose are prepared in tubes and concentrated viral proteins are layered over the gradient with good acceleration of RCF 1,048,000xg for 3 hrs. The separated proteins can be collected from particular specific gravity band.

  Applications
  User Instructions
For separation of • Virus/viral antigen/proteins • Lipoproteins • Nanoparticles • Nucleic Acids1. Sample must be pure and free from all contaminants 2. Concentrated sample volume should be small quantity to layer over gradient 3. Presently sucrose gradient is being used

  Contact Us
Contact No. 080-23093111
Email Id director.nivedi@icar.gov.in

  Ultracentrifuge with accessories + Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
1000
800
700

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""