Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Institute for Arid Horticulture, Sri Ganganagar Highway, Beechwal Industrial, Area Post Off. Bikaner-334006 Rajasthan (
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Instrument Details
Instrument/Facility Name: Atomic Absorption Spectrophotometer (AAS)
Make                                   : GBC Scientific
Model                                  : SENSAA SERIES AAS
Specification   :Atomic Absorption Spectrophotometer

Click to ViewEnvironmental conditions: Air quality, Temperature range, Relative humidity, Altitude, Main supply: Frequency 50 to 60 Hz, Gases: Air, N2O, Acetylene, Connection: Gases, Power, Performance: In build computer: Hardware,(touch screen interface computer, Pentium Or AMD CPU, Minimum 256MB RAM, One parallel port ,four USB ports,40 GB storage) software(Microsoft windows @ XP operating system),Data processing. (Absorption range 2.5) ,Graphics, (Numerical information from graphic traces), Data storage (signal graphics ,applications ,results, sample lables, weight and dilutions and report headers) Quality, (GLP and GMP) report generation, (Flame,furnance and vapour generation) Instrument: Optics, ( single beam (Sens AA) or double beam (Sens AA dual) monochromator (333 mm focal length and 175-900 nm wavelength range. ,Choice of lamp types, (Hollow cathode lamp or application source with vertical and horizontal adjustments) Lamp supply, background correction (Lamp provides 175-423nm correction to 2.5 total absorbance) ,Interlocked flame control( safety interlocks on burner, nebulizer, pressure relief bung and liquid trap level), Gas control modules (Microprocessor controlled) GCM (Optional), Flame atomization system (Solid polypropylene mixing chamber), Wight and dimensions: weight (unpacked 48kg,packed 60 kg), Spectrometer module: 830x580x520(WXDXH),Footprint(455mmx810mm). Atomic Absorption Spectrophotometer (AAS) is a laboratory instrument used for measuring the concentration of micronutrients in soil, plant, water, food products and fertilizers in sample solutions. This instrument can measure the concentration of elements up to parts per million (ppm) in any sample using flame mode and up to parts per billion (ppb) using Graphite Furnace mode.

  Working Principles
Click to ViewAtomic absorption spectroscopy is a technique which studies absorption of electromagnetic radiations in relationship to molecular structure. It is a technique for measuring the concentration of various elements in the sample through their absorption of light. It is a relatively simple and reliable technique which uses absorption of optical radiation by free atoms for determining the contents of different elements.

  User Instructions
Atomic absorption spectroscopy has various applications in various fields. One of the major applications of atomic absorption spectroscopy is for the determination of structural elucidation of various substances. This technique is more beneficial as a very small quantity of substance is required for analyzing. Atomic absorption spectroscopy can be used to analyze water for its metal quantity if present. Atomic absorption spectroscopy is used in many pharmaceutical manufacturing processes where small quantities of catalyst remain in the final product. Atomic absorption spectroscopy can also be used in biological tissues such as blood, liver, brain tissue, muscle tissue and fluids for analyzing metals. In agriculture, it is used for the analysis of soils, plants, water and food materials etc. for measuring micronutrient contents. For extracting available micronutrients in soil, weigh 10 g ( 2 mm sieved) soil in 100 ml conical flask. Add 20 ml of DTPA extractant (0.005 M DTPA +0.1M Triethanolamine ( TEA) + 0.01 M CaCl2 , pH.7.3) and shake for 2 h on a mechanical shaker. Filter the contents through Whatman No. 42 filter paper (for quick filtration use Whatman No.1),discarding first few drops. The filtrate can be used for measuring the micronutrients ( Fe, Mn Zn and Cu) using AAS. In case of plant samples, digest 0.5 or 1 g oven dried sample in tri-acid mixture (AR grade conc. HNO3: H2SO4 : HClO4 (10:1:4) on hot-plate (temperature 180-200 C) and filter through Whatman No. 42 filter paper, make up the volume and take reading using AAS.

  Contact Us
Contact No. 9460616489
Email Id

  Atomic Absorption Spectrophotometer (AAS) Charges
Procedure Name
National LAB/R&D
Standard Protocol of the elements analysis from soil samples
Zn, Fe, Mn & Cu only
Standard Protocol of the elements analysis from plant samples
Zn, Fe, Mn & Cu only

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT - A/C, CIAH Bikaner"
Letter, DD & Samples send to "Director, ICAR-CIAH, 10 Km milestone Sriganganagar Highway, Beechwal Bikaner Rajasthan-334006"