Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Research Institute for Dryland Agriculture, Santoshnagar, Saidabad PO, Hyderabad 500059 Telangana (
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Instrument Details
Instrument/Facility Name: Atomic Absorption Spectrophotometer (AAS)
Make                                   : Perkin Elmer, USA
Model                                  : Model –AAnalyst 800 + THGA Graphite Furnace
Specification   :Photometer with real time double-beam optical system (single-beam for Zeeman furnace operation with the A-Analyst 800). Front-surfaced, reflecting optics with protective coating. Monochromator: Littrow design with motorized drive for automatic wavelength selection and peaking. Wavelength range: 190 - 870 nm. Diffraction grating: 1800 lines/mm blazed at 236 nm and 597 nm. Focal length: 267 mm. Spectral bandwidths:0.2, 0.7 and 2.0 nm, dual height; motorized slit drive for automatic slit selection.

Click to ViewAtomic Absorption Spectrophotometer (AAS) is a laboratory instrument used for measuring the concentration of micronutrients in soil, plant, water, food products and fertilisers in sample solutions. This instrument can measure the concentration of elements up to parts per million (ppm) in any sample using flame mode and up to parts per billion (ppb) using Graphite Furnace mode.

  Working Principles
Click to ViewAtomic absorption spectroscopy is a technique which studies absorption of electromagnetic radiations in relationship to molecular structure. It is a technique for measuring the concentration of various elements in the sample through their absorption of light. It is a relatively simple and reliable technique which uses absorption of optical radiation by free atoms for determining the contents of different elements.

  User Instructions
Atomic absorption spectroscopy has various applications in various fields. One of the major applications of atomic absorption spectroscopy is for the determination of structural elucidation of various substances. This technique is more beneficial as a very small quantity of substance is required for analyzing. Atomic absorption spectroscopy can be used to analyze water for its metal quantity if present. Atomic absorption spectroscopy is used in many pharmaceutical manufacturing processes where small quantities of catalyst remain in the final product. Atomic absorption spectroscopy can also be used in biological tissues such as blood, liver, brain tissue, muscle tissue and fluids for analyzing metals. In agriculture , it is used for the analysis of soils, plants, water and food materials etc. for measuring micronutrient contents.For extracting available micronutrients in soil, weigh 10 g ( 2 mm sieved) soil in 100 ml conical flask. Add 20 ml of DTPA extractant (0.005 M DTPA +0.1M Triethanolamine ( TEA) + 0.01 M CaCl2 , pH.7.3) and shake for 2 h on a mechanical shaker. Filter the contents through Whatman No. 42 filter paper (for quick filtration use Whatman No.1),discarding first few drops. The filtrate can be used for measuring the micronutrients ( Fe, Mn Zn and Cu) using AAS. In case of plant samples, digest 0.5 or 1 g oven dried sample in tri-acid mixture (AR grade conc. HNO3: H2SO4 : HClO4 (10:1:4) on hot-plate (temperature 180-200 C) and filter through Whatman No. 42 filter paper, make up the volume and take reading using AAS.

  Contact Us
Contact No. +91-040 24532243, 24530161
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  Atomic Absorption Spectrophotometer (AAS) Charges
Procedure Name
National LAB/R&D
Atomic Absorption Spectroscopy
Per Sample Per Element for Soil
Per Sample Per Element for Plant

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT - A/C, CRIDA, Hyderabad"
Letter, DD & Samples send to "Director, ICAR-CRIDA, Santoshnagar, Hyderabad - 500 059, Telangana"