Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Tobacco Research Institute, Dr. N.C. Gopalachari Road, Bhaskar Nagar, Rajamundry-533105 , Andhra Pradesh (
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Instrument Details
Instrument/Facility Name: Atomic Absorption Spectrophotometer-CTRI
Make                                   : Perkinelmer, USA
Model                                  : AA700, 2006
Specification   :Monochromator: Littrow design with motorized drive for automatic wavelength selection and peaking, Wavelength range : 190 - 900 nm; Diffraction grating: 1800 lines/mm blazed at 236 nm and 597 nm; Grating area : 64 x 72 mm; Reciprocal linear dispersion: 1.6 nm/mm (nominal), Focal length: 267 mm; Spectral bandwidths : 0.2, 0.7 and 2.0 nm, dual height; motorized slit drive for automatic slit selection

Click to ViewAtomic Absorption Spectrophotometer (AAS) is a spectroanalytical instrument for the quantitative determination of chemical elements using the absorption of optical radiation (light) by free atoms in the gaseous state. In analytical chemistry the instrument is used for determining the concentration of a particular element (the analyte) in a sample to be analyzed. AAS can be used to determine over 70 different elements in solution or directly in solid samples.

  Working Principles
Click to ViewThe technique makes use of absorption spectrometry to assess the concentration of an analyte in a sample. It requires standards with known analyte content to establish the relation between the measured absorbance and the analyte concentration and relies therefore on the Beer-Lambert Law. In short, the electrons of the atoms in the atomizer can be promoted to higher orbitals (excited state) for a short period of time (nanoseconds) by absorbing a defined quantity of energy (radiation of a given wavelength). This amount of energy, i.e., wavelength, is specific to a particular electron transition in a particular element. In general, each wavelength corresponds to only one element, and the width of an absorption line is only of the order of a few picometers (pm), which gives the technique its elemental selectivity. The radiation flux without a sample and with a sample in the atomizer is measured using a detector, and the ratio between the two values (the absorbance) is converted to analyte concentration or mass using the Beer-Lambert.

  User Instructions
Determination of following elements in Soil and Tobacco: • Copper • Zinc • Iron • Manganese 1. Clearly mention the name of the character for which analysis is required, 2. Provide minimum 0.5-1.0 kg leaf or 100-200g powdered tobacco, 3. Provide minimum 1Kg soil

  Contact Us
Contact No. 0883 2449871-74
Email Id

  Atomic Absorption Spectrophotometer-CTRI Charges
Procedure Name
National LAB/R&D
For Copper
For Zinc
For Iron
For Manganese

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-CTRI, Rajahmundry"
Letter, DD & Samples send to "DIRECTOR, ICAR-CTRI, Bhaskar Nagar, Rajahmundry - 533105 Andhra Pradesh, India "