Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Indian Institute of Spices Research, Marikunnu P.O., Kozhikode-673012, Kerala (
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Gas chromatograph with head space
Make                                   : Perkin Elmer
Model                                  : Clarus 500
Specification   :Headspace-GC is used for qualitative or quantitative analysis of volatile components in samples that can be efficiently partitioned into the headspace gas volume from either a liquid or solid matrix.

Click to ViewGC Head space with FID Nitrogen generator Hydrogen generator Zero air generator Oven Temperature range: 10 °C above ambient to 300°C Column overheat protect: User settable up to 300°C. Oven temperature range : 45°C to 300°C and increment 1°C Detector: Flame Ionization Detector (FID)

  Working Principles
Click to ViewA headspace sample is normally prepared in a vial containing the sample. Volatile components from complex sample mixtures can be extracted from non-volatile components and further isolated in the headspace or vapor portion of a sample vial. An aliquot of the vapor in the headspace is delivered to a GC system for separation of all of the volatile components. The components in the vapor passes through a column and separation takes place on the basis of preferential adsorption. The separated compounds are detected using a FID detector.

  User Instructions
HS-GC is widely used in the detection of: Residual solvents in pharmaceuticals, food, packing material, aluminium and plastic films Flavour compounds in beverages and spices Odour compounds in foodstuffs, herbs, flowers and perfumes Rancidity of fat and oil Analysis of beverages for diketones (in beer), sulfur compounds, alcohols, esters, aldehydes and acids Ethanol in blood, food and beverages Volatile fermentation products from anaerobic bacteria. Methyl esters of fatty acids of vegetable oils Analyses of volatiles from leaf samples and estimation of residual solvents from samples may be undertaken.

  Contact Us
Contact No. 0495-2731410
Email Id

  Gas chromatograph with head space Charges
Procedure Name
National LAB/R&D

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit-IISR, Calicut"
Letter, DD & Samples send to "Head, SAIF, ICAR-Indian Institute of Spices Research, Marikunnu P.O., Kozhikode - 673012, Kerala."