Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Inland Fisheries Research Institute, PO Monirampare, Barrackpore, Kolkata-700120 West Bengal (
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Instrument Details
Instrument/Facility Name: HPLC
Make                                   : Waters
Model                                  : 1525 series
Specification   :

Click to ViewHPLC Waters 1525 series

  Working Principles
Click to ViewHPLC is a chromatographic technique used to separate a mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying, quantifying or purifying the individual components of a mixture. A HPLC system has basically a stationary phase (column) through which the mobile phase carrying the sample is passed and the analytes (compounds to be separated) are eluted from the column depending upon their chemical structure and interaction with the column. Different components of a complex mixture get separated in the column due to various physico-chemical interactions with the column. HPLC has many uses including medical diagnosis (e.g. detecting vitamin D levels in blood serum), legal (e.g. detecting performance enhancement drugs in urine), research (e.g. separating the components of a complex biologicalsample, or of similar synthetic chemicals from each other), and manufacturing (e.g. during the production process of pharmaceutical and biological products) and quality control (quantification of certain compounds in a product).

  User Instructions
Amino acid analysis of biological samplesWet tissue samples/blood/serum/plasma etc.should be brought in ice/dry ice. Dry samples like dried tissues samples and grains can be brought wrapped in aluminum foils or plastic bags.

  Contact Us
Contact No. 033 -2545 2061(O)
Email Id -

  HPLC Charges
Procedure Name
National LAB/R&D

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "-"
Letter, DD & Samples send to "-"