Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Tobacco Research Institute, Dr. N.C. Gopalachari Road, Bhaskar Nagar, Rajamundry-533105 , Andhra Pradesh (
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Instrument Details
Instrument/Facility Name: High Performance Liquid Chromatography
Make                                   : SHIMADZU, JAPAN
Model                                  : LC-8A, 1995
Specification   :Flow rate accuracy: 2% (0.5-150 mL/min); Flow rate stability: 0.5% (0.5-150 mL/min); Wet parts material: Stainless steel, Hastelloy C, Vespel, Sappire; Suction filter: 10 µm; Dimensions (mm): 350(w) x 450(d) x 210(h)

Click to ViewHigh-Performance Liquid Chromatograph (HPLC) is an analytical instrument used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

  Working Principles
Click to ViewThe sample is being injected through analytical port into the mobile phase stream which carries it into the column. The pumps deliver the desired flow and composition of the mobile phase through the column. Chromatography can be described as a mass transfer process involving adsorption. The active component of the column, the adsorbent, is typically a granular material made of solid particles (e.g. silica, polymers, etc.), 2–50 μm in size. The components of the sample mixture are separated from each other due to their different degrees of interaction with the adsorbent particles. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor and user software control the HPLC instrument and provide data analysis.

  User Instructions
Determination of following parameters in Tobacco: • Solanesol • Imidacloprid 1. Clearly mention the name of the character for which analysis is required; 2. Provide minimum 0.5-1.0 kg leaf or 100-200g powder

  Contact Us
Contact No. 0883 2449871-74
Email Id

  High Performance Liquid Chromatography Charges
Procedure Name
National LAB/R&D
For Solanesol
For Imidacloprid

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-CTRI, Rajahmundry"
Letter, DD & Samples send to "DIRECTOR ICAR-CTRI, Bhaskar Nagar, Rajahmundry - 533105 Andhra Pradesh, India "