Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Indian Institute of Soybean Research, Near IT Park, Khandwa Road, Indore-452001, Madhya Pradesh (
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Instrument Details
Instrument/Facility Name: High Performance Liquid chromatography(HPLC)
Make                                   : Shimadzu
Model                                  : 10AT vp
Specification   :HPLC Pump (LC-10AT vp): Flow rate range- 0.001ml /min to 9.0ml/min Max. operating pressure- 400 kgf Manual Injector: Loop Size- 20 µl Autosampler (SIL-20A): Injection Volume Range- 0.1 to 100 µl Column Oven (CTO-10A vp): Temp. range- 4.0 to 80.0 áµ’C UV/VIS Detector (SPD-10A vp): wavelength range- 190 to 600 nm RID Detector (RID-10A): Refractive index range- 1 - 1.75 RIU Communication Bus Module (CBM-20A): System controller equipped with a data buffering function Acts as an interface for connecting LC workstations, network-client computers, and analytical instruments via Ethernet Software: LC Solution 9. Hardware: Computer system

Click to ViewHPLC instrument generally constitute of autosampler, pumps, and a detector. The autosampler injects specific volume of sample mixture into the mobile phase stream which carries it into the column. The pumps deliver the desired flow (isocratic or gradient) of the mobile phase through the column. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor (Communication Bus Module) and user software (LC Solution) control the HPLC instrument and provide data analysis. Available Accessories: 1. Mobile Phase Reservoir 2. Filtration Unit

  Working Principles
Click to ViewHPLC is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase and a mobile phase. The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of various components.

  User Instructions
Detection of sucrose and Isoflavones (diadzein and genistein)Users are required to bring following things by themselves 1. Column 2. Mobile Phase 3. Syringe 4. Syringe Filter (PVDF) 5. PVDF membrane filter for filtration unit 6. HPLC grade chemicals 7. HPLC grade Water

  Contact Us
Contact No. 0731-2437963(O), 9302106779(M)
Email Id,

  High Performance Liquid chromatography(HPLC) Charges
Procedure Name
National LAB/R&D

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""