Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Directorate of Groundnut Research, Ivnagar Road, P.B.No.5, Junagadh-362001 Gujarat (
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Instrument Details
Instrument/Facility Name: High performance liquid chromatography (HPLC)
Make                                   : Agilent Technology, USA
Model                                  : 1260 Infinity Quaternary LC
Specification   :1. Operating pressure range up to 600 bar 2. Gradients of up to 4 different solvents 3. Flow rate of 0.2 to 10 ml/min

Click to View• Quaternary gradient with flow range of 0.001 – 10.000 ml/min. • Maximum operating pressure of 5880 psi • C-18 column for aflatoxin analysis with guard columns and also specific column for aflatoxin detection (B1 and total) • Florescence detector with Kobra cell as post column derivatization for aflatoxin

  Working Principles
Click to ViewHigh performance liquid chromatography (HPLC) is basically a highly improved form of column liquid chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. That makes it much faster. All chromatographic separations, including HPLC operate under the same basic principle; separation of a sample into its constituent parts because of the difference in the relative affinities of different molecules for the mobile phase and the stationary phase used in the separation.

  User Instructions
• Analysis of aflatoxin B1, B2, G1 and G2 1. Minimum of five samples should be submitted 2. Provide minimum 200g sample per analysis

  Contact Us
Contact No. 0285-2673382
Email Id

  High performance liquid chromatography (HPLC) Charges
Procedure Name
National LAB/R&D
Aflatoxin(B1, B2, G1 and G2) analysis from groundnut kernel
per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""ICAR-Directorate of Groundnut Research, Junagadh OR Electronic transfer to ICAR-Directorate of Groundnut Research, Junagadh (ACC No: 10283132519 IFSC Code: SBIN0003251)""
Letter, DD & Samples send to ""Director, ICAR-Directorate of Groundnut Research, PB 5, Junagadh, Gujarat, India-362001""