Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh (
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Instrument Details
Instrument/Facility Name: Liquid Chromatography- Mass Spectrometer (LC-MS) system
Make                                   : M/s Waters
Model                                  : SQD2 with H-class UPLC
Specification   :

Click to ViewSingle Quadrupole Mass Spectrometer System has ESI and APCI as well as positive and negative modes of ionization Mass Range: 20 to 2000 m/z PDA Detector: Aquity UPLC PDAeʎ Detector Wavelength range: 200 nm to 600 nm Solvent Delivery System High pressure binary gradient pump with online degassing unit Mode of operation: Isocratic and gradient Flow rate range: 50 µl/min to 500 µl /min Injector and Autosampler with temperature control Temperature range: from 8 °C to 40 °C Injection volume range: 0.5 μl to 10 μl

  Working Principles
Click to ViewLiquid chromatography- Mass Spectroscopy (LC- MS) is a technique which combines high performance liquid chromatography (HPLC), a powerful analytical separation technique with mass spectroscopy (MS), a powerful analysis & detection technique. Electrospray Ionization (ESI) is a soft ionization technique most widely used in modern times MS and is compatible with most chromatographic separations. HPLC seperations can be detected by “Photo Diode Array (PDA)” detector and Mass spectrometer as different detectors. PDA has three dimensional projection over entire UV-VIS range. It is non-destructive detector so it can be put in series like HPL C-PDA Mass Spectrometer.

  User Instructions
Qualitative Analysis Quantitative Analysis Impurity Profiling Metabolite Studies Pharmacokinetics Pharma & biomedical applicationsSolvent for mobile phase should be mentioned or solubility of compound in volatile solvent should be given. LC of submitted samples indicating the operating conditions should be supplied along with the sample. Source of the compound should be mentioned. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there are no toxic sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless accompanied by MS-DS.

  Contact Us
Contact No. 0177-2625073
Email Id

  Liquid Chromatography- Mass Spectrometer (LC-MS) system Charges
Procedure Name
National LAB/R&D
Only MS with ESI
Per sample
LCMS with ESI for first peak
Per sample
HPLC with PDA detector
Per sample
HPLC + PDA detector + Mass Spectrometer
Per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR- Unit CPRI Shimla"
Letter, DD & Samples send to "The Director, ICAR-CPRI Shimla"