Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Tobacco Research Institute, Dr. N.C. Gopalachari Road, Bhaskar Nagar, Rajamundry-533105 , Andhra Pradesh (
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Instrument Details
Instrument/Facility Name: MASTER CYCLER
Make                                   : Eppendorf
Model                                  : Master cycler 5345000.515 with gradient option
Specification   :

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  Working Principles
Click to ViewThe polymerase chain reaction (PCR) is a laboratory technique for DNA replication that allows a “target” DNA sequence to be selectively amplified. PCR can use the smallest sample of the DNA to be cloned and amplify it to millions of copies in just a few hours.

  User Instructions
• Amplification of specified genomic regions • Analysis of allelic sequence variations • Cloning of genomic DNA or cDNA • Mutagenesis or modification of DNA • Assays for the presence of pathogens • Detection of mutations • Genetic fingerprinting of forensic samples • All the PCR components should be handled in icebox/cooler box • Short spin of PCR tubes with the components before loading in to the machine will ensure that the components to settle at the bottom of the tube.

  Contact Us
Contact No. 0883 2449871-74
Email Id

Procedure Name
National LAB/R&D

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-CTRI, Rajahmundry"
Letter, DD & Samples send to "DIRECTOR, ICAR-CTRI, Bhaskar Nagar, Rajahmundry - 533105 Andhra Pradesh, India ""