Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Central Marine Fisheries Research Institute, P.B.No.1603, Ernakulam North P.O., Abraham Madamakkal Road, Kochi-682018 Kerala (
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Instrument Details
Instrument/Facility Name: Protein Analyzer
Make                                   : FOSS, DENMARK
Model                                  : KJELTEC 2300
Specification   :

Click to ViewThe FOSS KJELTEC 2300 Protein Analyzer is a semi-automatic system comprising of a digestion unit and fully automatic distillation and iteration units. A total of 20 samples can be analyzed in a row. Analysis time: 3-5 minutes per sample.

  Working Principles
Click to ViewWorking Principle: The method consists of three basic steps: (i) digestion of the sample (ii) distillation and (iii) quantification of the ammonia by titration. The sample is heated with concentrated sulphuric acid, which decomposes the organic substance by oxidation to liberate the reduced nitrogen as ammonium sulphate. In this step potassium sulphate and copper sulphate are added as catalysts. Chemical decomposition of the sample is complete when the initially dark-coloured medium becomes clear and colourless. The solution is then distilled with 40 % sodium hydroxide, which converts the ammonium salt to ammonia which is trapped into a solution of 4 % boric acid. The amount of ammonia present, and thus the amount of nitrogen present in the sample, is determined by titration with a standard solution. Digestion: Sample + Con. H2SO4 → (NH4)2SO4(aq) + CO2(g) + SO2(g) + H2O(g) Distillation: (NH4)2SO4(aq) + 2NaOH → Na2SO4(aq) + 2H2O(l) + 2NH3(g) Capture of ammonia: NH3 + H3BO3 (boric acid) → NH4+ + H2BO3- Titration: H2BO3- + H+ → H3BO3 Applications: Estimation of crude protein content of fish tissues, raw materials and finished products in feeds

  User Instructions
Estimation of crude protein content of fish tissues, raw materials and finished products in feeds Sample size: Feed and feed ingredients - 250 g; Other samples – 100 g in air tight plastic container; Liquid material - 250 ml in leak proof container along with clear labels and marks over each sample.

  Contact Us
Contact No. 9496280584
Email Id

  Protein Analyzer Charges
Procedure Name
National LAB/R&D
Dr. Vijayagopal

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""