Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Sugarcane Breeding Institute, Veerakeralam, Coimbatore-641007, Tamil Nadu (
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Instrument Details
Instrument/Facility Name: Real Time PCR
Make                                   : Applied Biosystems, CA, USA
Model                                  : StepOnePlusâ„¢ Real-Time PCR
Specification   :96 well format

Click to ViewThe StepOnePlusâ„¢ System supports many Real-Time PCR applications, including the following: SNP Genotyping, Gene Expression Analysis, MicroRNA Expression, Gene Detection, Viral Load Analysis

  Working Principles
Click to ViewReal-time PCR is an advanced form of the Polymerase Chain Reaction that maximizes the potential of the technique. It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR), and semi-quantitatively, i.e. above/below a certain amount of DNA molecules (semi quantitative real-time PCR). Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA (like SYBR), and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence (like TaqMan).

  User Instructions
There are numerous applications for quantitative polymerase chain reaction in the laboratory. It is commonly used for both diagnostic and basic research. •Quantification of gene expression •Diagnostic uses •Detection of phytopathogens, GMOs •Genotyping Reactions should be prepared by the user with their own consumables.

  Contact Us
Contact No. 0422 - 2472621
Email Id

  Real Time PCR Charges
Procedure Name
National LAB/R&D
Sophisticated Analytical Instrument (Research Microscope – Plant Pathology Lab) – Services- reg.
Rs. 1000/r
Rs. 1000/r
Rs. 1000/r

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, Sugarcane Breeding Institute, Coimbatore "
Letter, DD & Samples send to "The Director, Sugarcane Breeding Institute, Coimbatore"