Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)

ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500030, Talangana (
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Instrument Details
Instrument/Facility Name: Ultra performance liquid chromatography (UPLC)
Make                                   : Waters
Model                                  : Acquity H class
Specification   :UPLC with PDA detector. Facilitate to Lignans estimation in sesame

Click to ViewACQUITY UPLC H-Class System is a streamlined system that brings together the flexibility and simplicity of quaternary solvent blending and a flow-through-needle injector to deliver the advanced performance expected of UPLC type separations – high resolution, sensitivity, and improved throughput – while maintaining the robustness and reliability. The results are obtained using ‘Empower’ software of UPLC

  Working Principles
Click to ViewThe UPLC is based on the principle of use of stationary phase consisting of particles less than 2 μm (while HPLC columns are typically filled with particles of 3 to 5 μm). The under lying principles of this evolution are governed by the Van Deemter, which is empirical formula that describes the relationship between flow rate and and plate height (HETP or column efficiency). H = A+B/ν+Cν

  User Instructions
In our laboratory it is mainly used for lignans estimation in sesameIt is software controlled and handled by Institute personnel only

  Contact Us
Contact No. 040-24598158
Email Id

  Ultra performance liquid chromatography (UPLC) Charges
Procedure Name
National LAB/R&D
Lignans estimation by UPLC

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""